1. Enwere kristal dị elu na-arụ ọrụ na cell conductivity.
Ngwọta: Mgbe ihicha cell conductivity na 1: 1 nitric acid, kpochaa ya na mmiri deionized.
2. Onye elu anaghị adị ọcha.
Ngwọta: ịgbanwe eluent.
3. Kọlụm chromatographic na-amịkọrọ ihe ndị na-eme ihe dị elu.
Ngwọta: Wash kọlụm ugboro ugboro na ọzọ na eluent na mmiri.
4. Nhọrọ ezighi ezi nke nha nha
Mgbe ị na-eme nyocha ion dị mma, ebe ọ bụ na nzụlite eluate dị oke elu, nhọrọ nke nha nha dị ala ga-eduga n'igosi oke uru conductivity.Naanị họrọ nha nha ọzọ.
5. Onye suppressor anaghị arụ ọrụ
Ngwọta: Lelee ma agbanyere onye nkpuchi.
6. Nleba anya nlele dị oke elu.
Ngwọta: Ghichaa ihe atụ.
1. Enwere afụ na mgbapụta.
Ngwọta: valvụ na-atọpụ iyuzucha valvụ nke mgbapụta mgbapụta, afụ na-agwụ ike.
2. A na-emetọ valvụ nke mgbapụta ma ọ bụ mebie ya.
Ngwọta: Gbanwee valvụ nlele ma ọ bụ tinye ya n'ime 1: 1 ngwọta nitric maka nhicha dị elu.
3. A na-emerụ ihe nzacha na karama eluent ma ọ bụ gbochie ya.
Ngwọta: Dochie nzacha.
4. Ezughị oke degassing nke eluent.
Ngwọta: Dochie eluent.
Ngwọta: Nyochaa ebe mgbochi n'akụkụ ụzọ na-asọpụta n'aka iji chọpụta ma kpochapụ ihe mgbochi ahụ.
1. A na-egbochi akpụkpọ ahụ nzacha kọlụm.
Ngwọta: Wepu kọlụm ma kpọghee njedebe mbata.Wepụ efere sieve nke ọma, tinye ya na 1: 1 nitric acid ma saa ya na ultrasonic wave maka nkeji 30, wee kpochaa ya na mmiri deionized ma gbakọta ya azụ, tụgharịa chịkọta chromatograph maka ịsacha.Rịba ama na chromatograph enweghị ike ijikọ ya na ụzọ eruba.
2. A na-egbochi valve injection ụzọ isii.
Ngwọta: Nyochaa ebe mkpọchi n'akụkụ ụzọ mgbaba n'aka iji chọpụta na nchọpụta nsogbu.
3. A na-egbochi valvụ nlele nke mgbapụta.
Ngwọta: Gbanwee valvụ nlele ma ọ bụ tinye ya n'ime 1: 1 ngwọta nitric maka nhicha dị elu.
4. A na-egbochi ụzọ mgbapụ.
Ngwọta: chọpụta ebe mgbachi dị ka usoro mkpochapụ nke nta nke nta wee mee nnọchi.
5. Oke ọsọ.
Ngwọta: Gbanwee mgbapụta ahụ na ọnụego kwesịrị ekwesị.
6. A na-edozi nrụgide kachasị elu nke mgbapụta ahụ dị ala.
Ngwọta: N'okpuru ọrụ na-arụ ọrụ nke kọlụm chromatographic, mezie nrụgide kachasị elu ka ọ bụrụ 5 MPa karịa nrụgide ọrụ ugbu a.
1. The ngwaọrụ anaghị agba ọsọ ruo ogologo oge zuru ezu dị ka zubere.
Ngwọta: Infusion na-aga n'ihu nke eluent ruo mgbe akụrụngwa kwụsiri ike.
2. Enwere afụ na mgbapụta.
Ngwọta: valvụ na-atọpụ iyuzucha valvụ nke mgbapụta mgbapụta, afụ na-agwụ ike.
3. A na-egbochi ihe nzacha nke mmiri ntinye mmiri nke mgbapụta ahụ, na-emepụta nrụgide na-adịghị mma n'okpuru ike nsị na ịmepụta egosipụta.
Ngwọta: Dochie nzacha ma ọ bụ tinye nzacha n'ime 1: 1 1M nitric acid a ga-asacha 5min na ultrasonic bat.
4. Enwere afụ na kọlụm.
Ngwọta: Jiri eluent kwadebere site na mmiri deionized iji sachaa kọlụm na obere ọsọ iji wepụ ihe ndị ahụ.
5. Enwere afụ na ụzọ eruba.
Ngwọta: Wepu kọlụm na ikpochapụ site na mmiri.
6. E nwere egosipụta na cell conductivity, nke na-eme ka mgbanwe mgbe niile nke baseline.
Ngwọta: sel na-agbapụta conductivity, afụ na-agwụ ike
7. Voltaji na-ejighị n'aka ma ọ bụ na-egbochi ya na static electrostatic.
Ngwọta: Tinye voltaji stabilizer na ala ngwa.
1. Oge tupu ikpo ọkụ nke ngwaọrụ ezughị ezu.
Ngwọta: Gbatịkwuo oge ikpo ọkụ.
2. Mgbapu mmiri.
Ngwọta: chọpụta ebe ntapu ma dozie ya, ọ bụrụ na enweghị ike idozi ya, dochie nkwonkwo ahụ.
3. Voltaji na-ejighị n'aka ma ọ bụ na-egbochi ya na static electrostatic.
Ngwọta: Tinye voltaji stabilizer na ala ngwa.
1. Ntinye uche nke eluent adịghị mma.
Ngwọta: Họrọ itinye uche nke ọma.
2. Ọnụ ego nke eluentis dị oke elu.
Ngwọta: Họrọ ọnụego mgbaba nke eluent kwesịrị ekwesị.
3. Iji samples na oke itinye uche
Ngwọta: Ghichaa ihe atụ.
4. A na-emerụ kọlụm.
Ngwọta: Megharịa ma ọ bụ dochie kọlụm.
1. Olu injection nke ihe nlele ahụ anaghị adịgide adịgide.
Ngwọta: Tinye sample na olu karịa ugboro 10 nke ọnụọgụ mgbanaka iji hụ na ntụtụ zuru oke.
2. Nleba anya nke sample injected adịghị mma.
Ngwọta: Họrọ nleba anya nke ọma nke nlere ọgwụ.
3. Reagent adịghị ọcha.
Ngwọta: Dochie reagent.
4. Ihe ndị mba ọzọ dị na mmiri deionized.
Ngwọta: Dochie mmiri deionized.
5. The eruba mgbanwe.
Ngwọta: Chọpụta ihe kpatara mgbanwe ndị dị otú ahụ ma dozie ya na ọnọdụ mbụ.
6. A na-egbochi ụzọ eruba.
Ngwọta: chọpụta ebe akpọchiri, rụzie ma ọ bụ dochie.
1. The reagent adịghị ọcha.
Ngwọta: Dochie reagents.
2. Mmiri deionized nwere adịghị ọcha.
Ngwọta: Dochie mmiri deionized.
1. Ntinye na-ezighi ezi nke cell conductivity.
Ngwọta: Tinyegharịa cell conductivity.
2. Conductivity conductivity cell mebiri emebi.
Ngwọta: Dochie cell conductivity.
3. Mgbapụta ahụ enweghị ngwọta mmepụta.
Ngwọta: Lelee ihe nrụgide iji gosi ma mgbapụta na-arụ ọrụ.
1. A na-emerụ ihe ngwọta ọkọlọtọ, karịsịa obere ntinye uche.
Ngwọta: Kwadebe ọzọ ngwọta.
2. Mmiri e deionized adịghị ọcha.
Ngwọta: dochie mmiri deionized.
3. Nleba anya nke ihe nlele ahụ dị oke elu ma ọ bụ dị ala, site na ntanetị nke ngwaọrụ ahụ.
Ngwọta: Họrọ oke itinye uche nke ọma.
Ngwọta: Dochie eriri ọkụ ma ọ bụ ọkụ ọkụ dị ugbu a mgbe niile.
1. Na-amịkọrọ gas na ọkpọkọ ụzọ na-asọpụta
Ngwọta: mgbe a na-enye mmiri, mepee valvụ iyuzucha nke mgbapụta ahụ, malite mgbapụta plunger ma na-ama jijiji mgbe niile na nzacha iji wepụ gas ahụ kpamkpam.
2. Oke okpomọkụ dị n'ime ụlọ na-eduga na-ezughị ezu degassing nke deionized mmiri.
Ngwọta: Jiri ngwaọrụ ikuku ikuku.
3. A na-emetọ valvụ nke mgbapụta ma ọ bụ mebie ya.
Ngwọta: Gbanwee valvụ nlele ma ọ bụ tinye ya n'ime 1: 1 ngwọta nitric maka nhicha dị elu.